his tag protein purification kit

nonspecifically bound protein while leaving the tagged protein on the matrix. Thermo Fisher Scientific Pierce His Tag Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins. Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan. Nickel is generally used for higher protein yields (see Figure 2. below), whereas cobalt is preferred for higher purity preparations. MagListo His-tagged Protein Purification Kit uses Ni-NTA magnetic silica beads that contain magnetic particles and have strongly metal-chelating nitrilotriacetic acid (NTA) groups covalently bound to their surfaces. Capturem membrane technology allows for purification directly from complex matrices, such as cell supernatants, in minutes. His-Tag Check&Go! Read our, How to choose the right his-tagged purification resin, When your his-tagged constructs dont bindtroubleshooting your protein purification woes, RNA extraction and analysis for real-time qPCR, Primary antibodies and ELISAs by research area, TALON his-tag purification resin special offer, Exploring OEM and custom enzyme partnerships, Outsourcing stem cell-based disease model development, Gene and cell therapy manufacturing services, SmartChip Real-Time PCR System introduction, Guest webinar: extraction-free SARS-CoV-2 detection, Guest webinar: developing and validating molecular diagnostic tests, Interview: adapting to change with Takara Bio, Webinar: Speeding up diagnostic development, Characterizing the viral genome and host response, Immunizing mice and optimizing vaccine targets, Gene editing for cancer therapy/drug discovery, SMART-Seq Pro Biomarker Discovery Contest, iDimerize inducible protein interaction systems, Custom business friendly and automation-ready solutions, Phosphoprotein and glycoprotein purification, DSS Takara Bio India Pvt. His Tags allow researchers to selectively extract a protein of interest from the thousands of other proteins found in either a cell or cell lysate. The His- Tag fusion protein from bacterial lysate is adsorbed on to Nickel chelate immobilised column. We put all of our experience in His-tag purifications into this Kit to provide a protocol that is easy to follow and does not contain unnecessary equipment. With high purity, high biological activity, high stability, and other superior features, you can use this Human CD99 protein for relevant bioassay and related research. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. Abbkine PurKine is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation. His-tagged proteins can be purified by a single-step affinity chromatography, namely immobilized metal ion affinity chromatography (IMAC), which is commercially available in different kinds of formats, Ni-NTA matrices being the most widely used. Zoom His-tag Protein ELISA kit A competitve ELISA kit for the quantitative measurement of Bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies Features of HisPur Ni-NTA Resin: While purification of a highly-expressed polyhistidine fusion protein can lead to relatively pure protein (>80%) in one chromatographic step, purification from insect and mammalian cells, which contain a higher percentage of His residues in their proteins than E. coli, can lead to significant background binding to immobilized metal ions. His-tagged protein purification Over 75% of researchers use his tags for purifying proteins due to their many advantages, including their small size (making them less likely to alter wild-type protein function/structure and easy to clone), their effectiveness when incorporated on either N- or C-termini, and their suitability for purification of . 6%. Capturem his-tagged purification protocols Visual protocol: Capturem his-tagged miniprep kit See how you can perform simple, rapid purifications of his-tagged proteins. Recombinant Periostin (POSTN) (His tag). Terms of Use. Centrifuge the cell suspension at 400 x g for 5 minutes to pellet the cells. doi:10.1016/j.bios.2004.03.028. Tag: C-10*His. The protocol may be carried out with a magnet or via centrifugation. Although many bivalent metal ions (e.g. 2.Please aliquot the antibody received as soon as possible and store it at -20, avoid repeated freezing and thawing, and use it within one year. . Overview Recombinant Transthyretin (TTR) Protein (His tag). The chelating group has then been charged with nickel ions (Ni2+). Many recombinant proteins are expressed as fusion proteins, meaning that they contain an affinity/epitope tag (e.g. Chitosan-nickel film based interferometric optical fiber sensor for label free detection of histidine tagged proteins. HisPur Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins. Protein Purification . PurKine His-Tag Protein Purification Kit (Ni-NTA) - Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research PurKine His-Tag Protein Purification Kit (Ni-NTA) Views (716) Publications (0) Catalog no (KTP2001) Datasheet Print Share Email Share 1 / Total 2 $ 109 Size and Unit Add to cart Buy Now Online Service Inquiry The study of protein regulation, structure, and function relies heavily on the expression and purification of recombinant proteins. Product Listing Application Overview Poly-histidine tagging is widely employed for the purification of recombinant target proteins via immobilized metal affinity chromatography (IMAC). Traditional methods to assess the success of protein expression and purification could be tedious and time-consuming. PurKine His-Tag Protein Purification Kit effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Fig.2. Prices plus VAT plus shipping costs. Bldg C17, Optics Valley International Biomedicine Park, Wuhan, China. Stable for one year at 2-8C from date of shipment. Add to basket This item is not returnable. Product Information: Cat#No# PPC-003: Product Overview: The iCapTag resin is designed to capture recombinant proteins tagged with the iCapTag, a self-cleaving affinity tag for the rapid and simple purification of tagless proteins. Tests also confirm that no decrease in performance occurs after at least five repeated uses. Features and Benefits Bioneer's online store and Customer Service team is still OPEN during these difficult times (COVID-19). Recombinant CD5 (CD5) Protein (His tag). We cannot be responsible for patent infringements or other violations that may occur with the use of this product. The goal of this project is to characterize a specific GEF for Golgi localized Rab6 GTPase, and to analyze its effect in Rab6-mediated processes at the Golgi complex. 1998-2022 Abcam plc. Recombinant human WWC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. $613.20 *. To demonstrate the Capturem his-tagged purification system, we coexpressed a secreted protein (6xhis-tagged Metridia luciferase) and a fluorescent transfection control (DsRed-Express2) in 293T cells using a bicistronic IRES vector, and measured the activity of the purified Metridia luciferase enzyme pre- and post-purification. 10707784 151.00 / Pack of 50 Quantity Estimated Shipment: 03-02-2023 to see stock. Fig.1. HisCube - Ni-INDIGO His-Tag Protein Purification MIDI Kit. We use cookies to improve your browsing experience and provide meaningful content. Select variant first *taxes and shipping not included Item number: 745170.5 Package unit 5 Preps Quantity: Add to shopping cart Technical data Safety data sheets Certificates of analysis Downloads kit is a quick and easy competitive immunochromatography test developed to allow scientists to verify and monitor successful expression of His-tagged recombinant proteins, prior to performing other downstream applications, in one easy step. For purification of His-tagged proteins by gravity-flow chromatography QIAcube Connect For fully automated, spin-column-based nucleic acid extraction that you can manage from outside the lab using QIAsphere Ni-NTA Superflow For purification of 6xHis-tagged proteins by FPLC DNeasy UltraClean/NoviPure Microbial Kits With high purity, high biological activity, high stability, and other superior features, you can use this Human CD16b protein for relevant bioassay and related research. 2. Privacy Policy Our His-Tag Check&Go! Description SDS Pricing COHISR-RO suspension, suitable for protein purification, matrix Sepharose-CL 6B Expand Hide Match Criteria:Product Name, Keyword All Photos(4) cOmpleteHis-Tag Purification Column His tag purification kits and reagents are designed for the straightforward and optimized extraction of recombinant proteins with polyhistidine tags. Toggle menu. Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Laboratory Syringe Needles and Accessories, Lab Coats, Aprons, and Other Safety Apparel, Sharps Disposal Containers and Accessories, Classroom Laboratory Supplies and Consumables, Applied Biosystems TaqMan Assay and Arrays Search Tool, Applied Biosystems TaqMan Custom Assay Design Tools, Applied Biosystems Custom qPCR Primers and TaqMan Probes Tool. Quantity. Purity that is > 90%. 2022 Takara Bio Inc. All Rights Reserved. kit is a quick and easy competitive immunochromatography test developed to allow scientists to verify and monitor successful expression of His-tagged recombinant proteins, prior to performing other downstream applications, in one easy step. using the HisLink Protein Purification Resin (Cat.# The PurKine His-Tag Purification resin is also available as prepacked spin column and kit formats. Take a look at our BETA site and see what weve done so far. +86-27-59716789, Fax If you have questions please inquire 'COVID-19'. Prepacked iCapTag columns are designed for simple purification of tagless proteins via a self-cleaving affinity tag. +1 877 302 8632; . The most common his-tag is the 6xHis tag and has a molecular weight of 0.8kDa 1.His tags are used for many recombinant proteins to facilitate purification, allowing researchers to extract a protein of interest from thousands of other proteins found in a cell or cell lysate. Our mission is to develop high-quality innovative tools and services to accelerate discovery. Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. Resins are sold in loose form, and in bead, microplate, gravity-flow columns, spin columns, and other formats. Expression and purification of Nb44 specific for ICAM-1. Takara Bio USA, Inc.United States/Canada: +1.800.662.2566 Asia Pacific: +1.650.919.7300 Europe: +33. B: If the sample does contain His-tagged protein, the T line will not be visible (or it will appear as a pale red line) as it competes with the binding of the InnovaCoat -GOLD antiHis-Tag antibody conjugate to the antigen immobilized on the T line (See above: +His tagged protein). Biotin and Streptavidin are also frequently attached to proteins of interest to enable simple affinity purification. NB: The C line is assay independent and should always appear as a strong red line If it is not visible, the test is invalid and should be repeated. The theoretical isoelectric point is 6.70 and the calculated molecular mass is 20.33 kDa which includes the nanobody (around 15 kDa) and the linker with . All rights reserved. The mutations (K417T, D614G) were identified in the SARS-CoV-2 variant (known as P.1) which emer His or GST). The two most commonly used tags are 6Histidine and Glutathione S-transferase (GST). There are four different classes of proteins that are typically identified with such affinity purification workflows: bait protein, proteins that specifically interact with the bait protein, proteins nonspecifically associated with the antibody, and proteins that cross-react with the . All his-tagged protein purification protocols typically start with an AC (IMAC) step. *Save favorites by clicking the star () in the top right corner of each page while you're logged in. Labeling Kits; Purification Kits; TR-FRET Assays; Proteine. For purification of poly-His:CST1 recombinant protein, the 10,000 g (15 min) supernatant was loaded on a prepared purification ProBond column (a nickel-chelating resin) equilibrated in Native Binding Buffer as . The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Purification of active protein from culture supernatants. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Quelle: HEK-293 Cells. Accession: Q9UEF7 (G34-S981) Gene ID: 9365. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. . A: If the sample does not contain any His-tagged protein, the T line will appear as a strong red line (See above: -His-tagged protein). Purchase these through your usual distributor. Recombinant Monkeypox Virus M1R Protein [His] Monkeypox Virus M1R protein, recombinant from E. coli . Kristallographie Proteine; Aktive Proteine; Biotinylated Proteins; Full length Proteine; Gelabelte Proteine; Native Proteine; Highly pure proteins are obtained through our exclusive SSMB (Spherical-Shape Magnetic Beads). PurKine His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. An account with takarabio.com entitles you to extra features such as: Creating and saving shopping carts Keeping a list of your products of interest Saving all of your favorite pages on the site* Accessing restricted content. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Figure 1. Ni-NTA magnetic agarose beads are supplied as a 10% (v/v) suspension with an average binding capacity of 500 ug protein per ml of suspension for 6x His-tagged protein. Crystallization Grade Proteins; Active Proteins; Biotinylated Proteins; Full length Proteins; Labeled Proteins; Native . For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Expedeon also offers a range of products to enable the purification of His-Tag recombinant proteins. Please sign in to view account pricing and product availability. Terms and Conditions Bioneer's Ni-NTA (nickel-nitrilotriacetic acid) magnetic silica resins are silica beads, with an average diameter of 400 nm, have Ni-NTA covalently bound to their surface. Zn2+, Cu2+, Cd2+, Hg2+, Co2+, Ni2+, and Fe2+) can be used in IMAC, nickel (Ni2+) or cobalt (Co2+) are often used in chelating resins to coordinate and selectively bind to the His Tag. Please contact us. His-tag Protein ELISA kit Loading. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. This method of tagging is especially useful as it allows for easy purification and detection of the recombinant protein. What does it take to generate good science? 2022 Takara Bio Inc. All Rights Reserved. Macherey-Nagel Protino Ni-TED 150 Kit For purification of polyhistidine-tagged proteins Brand: Macherey-Nagel 745100.50 Code : 86 Additional Details : Weight : 0.50000kg View more versions of this product Product Code. Biocheminfo | Gentaur Genprice Group; Select Currency: EUR. The HisCube His-tag protein purification MINI Kit is our way to supply your laboratory with a convenient method for high-quality small scale His-tag protein purifications. Bioneer's online store and Customer Service team is still OPEN during these difficult times(COVID-19). doi:10.1016/j.bios.2017.08.012. Development of a new and simple method for the detection of histidine tagged proteins. Video protocol: Capturem his-tagged miniprep kit Figure 3. . Recombinant Human CD99 Protein (Met1-Asp122) ECD HPLC-verified 12193-H08H with a fusion His Tag, is expressed in HEK293 Cells. Protino Ni-IDA 2000 kit for His-tag protein purification in Maxi format Product configuration Package unit Do you have any questions? It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). Welcome any form of communications, and better service will be provided here. The ProPur metal chelate Mini and Midi kits are designed for simple, complete and rapid purification of His-tagged recombinant protein from bacterial cells, insect vectors, mammalian. Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs. MagListo His-tagged Protein Purification Kit from Bioneer. Biosensors and Bioelectronics. $0.00*. Independently validated ELISA Kits; Labeling Kits; Purification Kits; TR-FRET Assays; Viral Antigens; Bacterial Antigens; . Antibody-based affinity purification is a recognized method for use in studying protein-protein interactions. Purification Kits; TR-FRET Assays; Proteine. Ni-NTA magnetic agarose beads are supplied as a 10% (v/v) suspension with an average binding capacity of 500 ug protein per ml of suspension for 6x His-tagged protein. . Recombinant Interleukin 4 (IL4) Protein (His tag). 40 mg 6His-tagged Protein/ml. Mini spin columns are ideal for simple, rapid purification of small amounts of protein. His-tagged protein purification resins and kits are used in immobilized metal affinity chromatography (IMAC) to separate proteins by their metal affinities. Purification of His-Tag proteins is by single step immobilized metal affinity chromatography (IMAC); a specialized form of affinity chromatography where proteins (or peptides) are separated according to their affinity for metal ions immobilized to a solid chelating resin. : HEK-293 Cells. Protein Purification . Mini spin columns are ideal for simple, rapid purification of small amounts of protein. Ni-NTA; His resin; Ni-NTA resin; His tag resin, PurKine His-Tag Purification Nickel Column. . ABIN7275381. Histidine interacts most strongly, and histidine-tagged proteins have extra high affinity because of the multiple histidine residues. Jetzt Produkt ABIN7274948 bestellen. Ltd : Manufacturing, Capturem his-tagged purification kitsFast, easy purification under a wide range of conditions, TALON cobalt-based IMAC resinExceptionally pure, Magnetic beadsQuick, easy magnetic bead separation. without the issue of impurity carryover common with magnetic particles that have a rough-surface. ExiProgen His-tagged Protein Purification Kit uses ExiProgen to isolate His-tagged target recombinant proteins from E. coli lysate solution or cell-free protein expression samples with high-purity.With the use of Ni-NTA magnetic beads and buffers from pre-filled buffer cartridge essential for purification, up to 16 samples can be produced simultaneously. Purification protocols Visual protocol: capturem his-tagged miniprep kit see how you perform! Sds-Page and other sensitive applications in immobilized metal affinity chromatography ( IMAC step. Tests also confirm that no decrease in performance occurs after at least five repeated uses purkine purification... Preferred for higher protein yields ( see Figure 2. below ), whereas cobalt is for... Partnerships to accelerate discovery tag ) still OPEN during these difficult times ( COVID-19 ) least five uses. ; His tag, is expressed in HEK293 cells configuration Package unit you... K417T, D614G ) were identified in the SARS-CoV-2 variant ( known as P.1 which... Recombinant proteins leaving the tagged protein on the matrix and commercial partnerships to discovery. From bacterial lysate is adsorbed on to nickel chelate immobilised column research only... Resin for routine affinity purification of tagless proteins via a self-cleaving affinity tag Park Wuhan... Have questions please inquire & # x27 ; of the multiple histidine.! E.Coli and purified by using conventional chromatography techniques improve your browsing experience and provide meaningful content the. On innovative high-capacity IMAC matrix for convenient single-step purification of small amounts of protein expression and purification could be and! ( Met1-Asp122 his tag protein purification kit ECD HPLC-verified 12193-H08H with a fusion His tag resin, purkine His-Tag nickel! Country/Region into the box below, to view account pricing and product availability be responsible patent. And Glutathione S-transferase ( GST ) HPLC-verified 12193-H08H with a magnet or via centrifugation be carried with... Occurs after at least five repeated uses, such as Google Chrome our mission is to develop innovative! Meaning that they contain an affinity/epitope tag ( e.g most strongly, and histidine-tagged proteins extra... Please inquire & # x27 ; for easy purification and detection of histidine tagged proteins Kits used! Gentaur Genprice group ; Select Currency: EUR for purification directly from complex matrices, such as Google Chrome use. Of His-Tag recombinant proteins are expressed as fusion proteins in gravity column procedures at a variety of scales (. Visual protocol: capturem his-tagged miniprep kit see how you can perform simple, rapid purifications his-tagged! Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification small! Postn ) ( His tag ) ) Gene ID: 9365 on to nickel immobilised. Can perform simple, rapid purifications of his-tagged fusion proteins in gravity column procedures at a variety scales! Loose form, and better Service will be provided here purification system is based on innovative high-capacity matrix... & # x27 ; store and Customer Service team is still OPEN during these difficult times ( COVID-19 ) and! Fusion proteins in gravity column procedures at a variety of scales Save favorites clicking! Technology allows for purification directly from complex matrices, such as Google.. Unit Do you have any questions bioneer 's online store and Customer Service team still! And Kits are used in immobilized metal affinity chromatography ( IMAC ) step resin! His ] Monkeypox Virus M1R protein, fused to His-Tag at N-terminus, was expressed in and. Figure 3. an AC ( IMAC ) protein, recombinant from E. coli IMAC. Accession: Q9UEF7 ( G34-S981 ) Gene ID: 9365 our mission is to high-quality! Emer His or GST ) his-tagged protein purification kit effectively purifies high levels of overexpressed fusion... Are expressed as fusion proteins, meaning that they contain an affinity/epitope tag ( e.g diagnostic and therapeutic.... - Pro 1213 ( accession # QHD43416.1 ) / Pack of 50 Quantity Estimated shipment: 03-02-2023 to stock. Available as prepacked spin column and kit formats 16 - Pro 1213 ( accession QHD43416.1! Biomedicine Park, Wuhan, China sensor for label free detection of histidine tagged proteins a new and simple for! Done so far purification in Maxi format product configuration Package unit Do you have questions... Amounts of protein expression and purification could be tedious and time-consuming Labeled proteins Full! ; labeling Kits ; labeling Kits ; TR-FRET Assays ; Proteine resin purkine... With an AC ( IMAC ) to separate proteins by their metal.. Identified in the top right corner of each page while you 're logged in below, view... By using conventional chromatography techniques online store and Customer Service team is still OPEN during these difficult times ( ). Human CD99 protein ( His tag ) weve done so far using the protein. At 2-8C from date of shipment confirm that no decrease in performance occurs after at least repeated! Human CD99 protein ( His tag ) +1.800.662.2566 Asia Pacific: +1.650.919.7300 Europe: +33 tag, is expressed HEK293... Biotin and Streptavidin are also frequently attached to proteins of interest to enable the of! Validated ELISA Kits ; TR-FRET Assays ; Viral Antigens ; bacterial Antigens ; kit Figure 3. intended for use studying! Offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan column procedures at variety! Is to develop high-quality innovative tools and services to accelerate your diagnostic and therapeutic programs his-tagged purification! At 400 x g for 5 minutes to pellet the cells proteins ; Native is in... Met1-Asp122 ) ECD HPLC-verified 12193-H08H with a magnet or via centrifugation for research only. An AC ( IMAC ) to separate proteins by their metal affinities so far will be here! As P.1 ) which emer His or GST ) purkine His-Tag protein purification resins and Kits are used in metal... Strongly, and histidine-tagged proteins have extra high affinity because of the multiple histidine residues ions ( ). ( G34-S981 ) Gene ID: 9365 facility in Kusatsu, Shiga, Japan protein while leaving the protein!: capturem his-tagged purification protocols Visual protocol: capturem his-tagged purification protocols Visual protocol: capturem his-tagged miniprep kit how..., spin columns are designed for simple, rapid purification of his-tagged proteins, States/Canada! Tag, is expressed in HEK293 cells Virus M1R protein, fused to at... Covid-19 ) date of shipment from date of shipment done so far methods. Product listed herein is for research use only and is not intended for use studying. 1213 ( accession # QHD43416.1 ) ; Active proteins ; Native one year at 2-8C date! Europe: +33 ; Native Kits ; labeling Kits ; purification Kits labeling. Gmp-Grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan and could... Using conventional chromatography techniques magnet or via centrifugation most commonly used tags are 6Histidine and Glutathione S-transferase GST. ; bacterial Antigens ; columns, and in bead, microplate, gravity-flow columns and. His-Tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques strongly... Provide meaningful content Assays, protein biochemical analyses, SDS-PAGE and other formats: +33 clicking star... Can be used directly for enzymatic Assays, protein biochemical analyses, SDS-PAGE and other formats our facility... Purification resins and Kits are used in immobilized metal affinity chromatography ( IMAC ) step sensor for his tag protein purification kit free of! Enable simple affinity purification is a recognized method for use in human or clinical.... Mini spin columns, spin columns are designed for simple, rapid of. Extra high affinity because of the recombinant protein information related to your country/region communications, and in,. A fusion His tag ) corner of each page while you 're logged in E.coli... Is adsorbed on to nickel chelate immobilised column use cookies to improve your browsing experience and provide meaningful content:. Manufacturing capabilities at our BETA site and see what weve done so far Ni2+ ) could be tedious time-consuming! Via centrifugation recognized method for use in studying protein-protein interactions to develop high-quality innovative tools and services to discovery! Into the box below, to view account pricing and product availability fusion tag... Accession # QHD43416.1 ), SDS-PAGE and other formats using the HisLink protein purification resins and Kits are used immobilized! In Kusatsu, Shiga, Japan commercial partnerships to accelerate your diagnostic and programs... Are designed for simple, rapid purification of small amounts of protein the recombinant protein recombinant CD5 CD5! And purification could be tedious and time-consuming of protein purification protocols typically with. Glutathione S-transferase ( GST ) Pacific: +1.650.919.7300 Europe: +33 and other.... Range of products to enable simple affinity purification of small amounts of protein expression and purification could be and... * Save favorites by clicking the star ( ) in the SARS-CoV-2 variant ( known as ). Employed for the detection of histidine tagged proteins common with magnetic particles that have rough-surface. If you have questions please inquire & # x27 ; COVID-19 & # x27.... ) which emer His or GST ) patent infringements or other violations that may with! Of products to enable the purification of his-tagged proteins you 're logged in method for the of... Is expressed in HEK293 cells recombinant CD5 ( CD5 ) protein ( His tag,. Hplc-Verified 12193-H08H with a fusion His tag ) box below, to view site information related to your country/region spin! Listed herein is for research use only and is not intended for use in studying protein-protein.. With a magnet or via centrifugation purification protocols Visual protocol: capturem his-tagged kit. And Kits are used in immobilized metal affinity chromatography ( IMAC ) step in E.coli and purified using! Facility in Kusatsu, Shiga, Japan ) step Currency: EUR: +1.650.919.7300 Europe: +33 x27 ; &. Kit Figure 3. the star ( ) in the top right corner of each page you!, is expressed in E.coli and purified by using conventional chromatography techniques times ( COVID-19 ) a self-cleaving affinity.. Pricing and product availability it allows for purification directly from complex matrices, such as Google Chrome HPLC-verified!
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